FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | lane2_Index12_1.fq |
| File type | Conventional base calls |
| Encoding | Illumina 1.5 |
| Total Sequences | 20868140 |
| Filtered Sequences | 0 |
| Sequence length | 90 |
| %GC | 49 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
![[WARN]](Icons/warning.png)
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AAAAA | 5314405 | 2.868638 | 5.1472373 | 2 |
| CCAGG | 4382055 | 2.6139395 | 5.546476 | 1 |
| CCTGG | 4386810 | 2.6035328 | 6.068274 | 1 |
| GAAGA | 4470935 | 2.553228 | 5.1484923 | 2 |
| CTTCT | 4555535 | 2.5297444 | 5.3647976 | 1 |
| AAGAA | 4506930 | 2.502286 | 5.191903 | 2 |
| CTGGA | 4220000 | 2.4505494 | 8.534795 | 1 |
| TCCAG | 4096445 | 2.3636675 | 5.2081747 | 2 |
| CTCCT | 4064515 | 2.3185298 | 6.9929442 | 1 |
| CTGGG | 3783545 | 2.259878 | 8.08731 | 1 |
| CAGGA | 3836300 | 2.2390666 | 5.5282226 | 1 |
| CTTCA | 3942510 | 2.20046 | 5.447266 | 1 |
| CCCAG | 3662430 | 2.170777 | 6.855912 | 1 |
| CTCCA | 3781195 | 2.1678863 | 7.2869315 | 1 |
| CAGAA | 3681210 | 2.0888631 | 5.125262 | 1 |
| CTCTG | 3533625 | 2.028599 | 6.1506605 | 1 |
| CTGGC | 3196745 | 1.8972399 | 5.2619214 | 1 |
| TGGAA | 3282235 | 1.8649081 | 5.1290326 | 2 |
| CTGAA | 3267080 | 1.8444877 | 5.551466 | 1 |
| CTCAG | 3009060 | 1.7362413 | 6.0319815 | 1 |
| CTGGT | 2903445 | 1.6774949 | 5.021926 | 1 |
| CTTGG | 2833795 | 1.6372536 | 5.2334485 | 1 |